Home - Current Protocols - Current Protocols comprises more than 17,000 regularly updated, peer reviewed, and indexed scientific techniques and procedures across 17 life science disciplines.

  • Categories - Current Protocols - Current Protocols Categories. Bioinformatics, Cell Biology, Chemical Biology, Cytometry, Essential Laboratory Techniques, Human Genetics, Immunology, MRI, Microbiology, Molecular Biology, Mouse Biology, Neuroscience, Nucleic Acid Chemistry, Pharmacology, Protein Science, Stem Cell Biology, Toxicology
  • Tools - Current Protocols - Current Protocols Scientific Calculators and Tools, Amino Acid Information Finder, Bacterial Media Recipes Calculator, Buffer Calculator, Colorimetric Assay Calculation Tool, Common Laboratory Recipes Calculator, DNA-Protein Translator Tool, DNA/RNA Base Composition Calculator, DNA/RNA/Protein Molecular Weight Calculator, G-Force/RPM Conversion Tool, Hemacytometer Calculation Tool, ImageJ Java Applet, NEBcutter v. 2.0, Nucleotide Information Finder, Polyacrylamide Gel Recipes Calculator, Primer3Plus, Radioactive Decay Calculator for Isotopes Commonly Used in Biomedical Research, Solution Concentration Calculator, Spectrophotometric Measurement of Nucleic Acids Calculator, Units of Measurement Conversion Tool
  • Titles - Current Protocols - Current Protocols Scientific Titles. Current Protocols in Bioinformatics, Current Protocols in Cell Biology, Current Protocols in Chemical Biology, Current Protocols in Cytometry, Current Protocols Essential Laboratory Techniques, Current Protocols in Human Genetics, Current Protocols in Immunology, Current Protocols in Magnetic Resonance Imaging, Current Protocols in Microbiology, Current Protocols in Molecular Biology, Current Protocols in Mouse Biology, Current Protocols in Neuroscience, Current Protocols in Nucleic Acid Chemistry, Current Protocols in Pharmacology, Current Protocols in Protein Science, Current Protocols in Stem Cell Biology, Current Protocols in Toxicolog
  • Pica in Rats as a Preclinical Model of Emesis - Current Protocols - The ability to assess the potential for gastrointestinal adverse events in a preclinical setting is a challenge in the development of new drugs, as the vast majority of in vivo research is conducted in rodent species lacking a vomiting reflex
  • Cerebral Cortex Electroporation to Study Projection Neuron Migration - Current Protocols - Brain electroporation is a rapid and powerful approach to study neuronal development
  • Cigarette Smoke Extract: A Preclinical Model of Tobacco Dependence - Current Protocols - Animal models are used to study many human diseases, one of which is tobacco addiction
  • The Neonatal Ventral Hippocampal Lesion (NVHL) Rodent Model of Schizophrenia - Current Protocols - Animal models are crucial to the study of the neurobiological bases of psychiatric disorders, but schizophrenia is a particularly challenging disorder to model given the complexity and heavily verbal nature of its symptoms
  • Automatic Dendritic Spine Quantification from Confocal Data with Neurolucida 360 - Current Protocols - Determining the density and morphology of dendritic spines is of high biological significance given the role of spines in synaptic plasticity and in neurodegenerative and neuropsychiatric disorders
  • Flow Analysis and Sorting of Plant Chromosomes - Current Protocols - Analysis and sorting of plant chromosomes (plant flow cytogenetics) is a special application of flow cytometry in plant genomics and its success depends critically on sample quality
  • Whole Blood Analysis of Leukocyte‐Platelet Aggregates - Current Protocols - In inflammatory and thrombotic syndromes, platelets aggregate with circulating leukocytes, especially monocytes and neutrophils
  • Automated Measurement of Blood Vessels in Tissues from Microscopy Images - Current Protocols - The quantification of tunica media thickness in histological cross sections is a ubiquitous exercise in cardiopulmonary research, yet the methods for quantifying medial wall thickness have never been rigorously examined with modern image analysis tools
  • Analysis of Heritability Using Genome‐Wide Data - Current Protocols - Most analyses of genome‐wide association data consider each variant independently without considering or adjusting for the genetic background present in the rest of the genome
  • Molecular Diagnosis of Myotonic Dystrophy - Current Protocols - Myotonic dystrophy types 1 (DM1) and 2 (DM2) are autosomal dominant, microsatellite repeat expansion disorders that affect muscle function
  • Pronuclear Injection‐Based Targeted Transgenesis - Current Protocols - Microinjection of DNA expression cassettes into fertilized zygotes has been a standard method for generating transgenic animal models
  • High‐Risk Screening of Fabry Disease: Analysis of Fifteen Urinary Methylated and Non‐Methylated Gb3 Isoforms Using Tandem Mass Spectrometry - Current Protocols - Fabry disease is a multisystemic, X‐linked lysosomal storage disorder caused by mutations in the GLA gene, leading to α‐galactosidase A deficiency and resulting in the accumulation of glycosphingolipids in different tissues and biological fluids
  • Acylglycine Analysis by Ultra‐Performance Liquid Chromatography‐Tandem Mass Spectrometry (UPLC‐MS/MS) - Current Protocols - Quantitative analysis of urine acylglycines has shown to be a highly sensitive and specific method with proven clinical utility for the diagnosis of several inherited metabolic disorders including: medium chain acyl‐CoA dehydrogenase deficiency, multiple acyl‐CoA dehydrogenase deficiency, short chain acyl‐CoA dehydrogenase deficiency, 3‐methylcrotonyl‐CoA carboxylase deficiency, 2‐methylbutyryl‐CoA dehydrogenase deficiency, isovaleric acidemia, propionic academia, and isobutyryl‐CoA dehydrogenase deficiency
  • Transcriptome Analysis at the Single‐Cell Level Using SMART Technology - Current Protocols - RNA sequencing (RNA‐seq) is a powerful method for analyzing cell state, with minimal bias, and has broad applications within the biological sciences
  • Strand‐Specific Transcriptome Sequencing Using SMART Technology - Current Protocols - Next‐generation sequencing is empowering a deeper understanding of biology by enabling RNA expression analysis over the entire transcriptome with high sensitivity and a wide dynamic range
  • Preparation of Low‐Input and Ligation‐Free ChIP‐seq Libraries Using Template‐Switching Technology - Current Protocols - Chromatin immunoprecipitation (ChIP) followed by high‐throughput sequencing (ChIP‐seq) has become the gold standard for mapping of transcription factors and histone modifications throughout the genome
  • Protein Interaction Profile Sequencing (PIP‐seq) - Current Protocols - Every eukaryotic RNA transcript undergoes extensive post‐transcriptional processing from the moment of transcription up through degradation
  • Isolation of Primary Fibroblast Culture from Wildlife: the Panthera onca Case to Preserve a South American Endangered Species - Current Protocols - Cell line establishment of somatic cells is a valuable resource to preserve genetic material of rare, difficult‐to‐find, endangered and giant species like Jaguar (Panthera onca), the largest South American felid
  • GILA, a Replacement for the Soft‐Agar Assay that Permits High‐Throughput Drug and Genetic Screens for Cellular Transformation - Current Protocols - For the last five decades, measuring the ability of cells to grow in soft agar has served as the gold standard assay for in vitro cellular transformation
  • Subscription and Purchasing Information - Current Protocols - How to purchase an institutional lab license to access current protocols' scientific protocols for life sciences procedures.

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